Roger

Dear M,

The delivery of drugs and supplements via suppository is a good idea indeed. Making a suppository is very easy:
– buy suppository molds – available online
– buy organic coconut oil
– warm up a little the coconut oil so that it becomes fluid, and after that mix the active ingredient at the concentration you like. Here you will need to check how much oil goes into one mold. If it goes 1ml oil, and you want to have a dose of 1000mg Curcumin in one suppository, you will need to make sure you mix this concentration in the oil. So if you want to prepare 100 suppositories at 1000mg each, you will need to mix 100g Curcumin in 100 ml coconut Oil (in this example)
– the mixture is injected into the suppository mold using a syringe
– the suppository molds containing the oil, need to be stored in the refrigerator overnight, before starting to use them – the coconut oil will become solid overnight and the next day, the suppository will be ready to be used.

Kind regards,
Daniel

亲爱的M，

通过栓剂递送药物和补充剂确实是一个不错的主意。制备栓剂非常简单：

• 购买栓剂模具 - 在线上有售
• 购买有机椰子油
• 将椰子油稍微加热，使其变成液体，然后与你喜欢的浓度的活性成分混合。在这里，你需要检查多少油放入一个模具中。如果1毫升油，你想在一个栓剂中有1000毫克的姜黄素，你需要确保在油中混合这个浓度。所以如果你想要准备每个1000毫克的100个栓剂，你需要在100毫升椰子油中混合100克姜黄素（在这个例子中）
• 使用注射器将混合物注入栓剂模具
• 含有油的栓剂模具需要在冰箱中存放一夜，开始使用之前 - 椰子油会在一夜之间凝固，第二天，栓剂将准备好供使用。

祝好， 丹尼尔

Hello,

What about suppositories like these (https://detoxrejuvenation.com/products/nexus-suppositories) to treat rectal cancer? These have garlic powder and are used for parasites. I have been looking for information for the preparation of suppositories but have yet to find specifics. Can anyone help? Many thanks.

M

你好，

对于治疗直肠癌，类似这种（https://detoxrejuvenation.com/products/nexus-suppositories）的栓剂如何呢？这些产品含有大蒜粉，并用于寄生虫。我一直在寻找有关制备栓剂的信息，但尚未找到具体细节。有人可以帮忙吗？非常感谢。

M

Hello,

I’ve been administering Artemisia annua (Luparte) to my dog who has disseminated histiocytic sarcoma and I was hoping to ask you for some clarification on your statement that “Artemisinin compounds inhibit their own absorption”. I’ve been researching this but I haven’t found anything useful and since the supplement manufacturers doesn’t recommend suspending the treatment to increase absorption, I was wondering if you have any references for this? The treatment doesn’t seem to be very effective until now, so I’m trying to figure out if we’re doing everything correctly…

Thank you!
Claudia

您好，

我一直在给患有弥漫性组织细胞肉瘤的狗（名为Luparte）使用青蒿素（Artemisia annua），我希望就您提到的“青蒿素化合物抑制其自身吸收”的说法向您请教一些澄清问题。我一直在进行研究，但没有找到任何有用的信息，由于补充剂制造商不建议中断治疗以增加吸收，我想知道您是否有任何参考资料？到目前为止，这种治疗似乎并不是很有效，所以我想弄清楚我们是否做得正确...

谢谢！
Claudia

HI Mihaela,

Thank you. I did found people willing to help even if the goal is to have most profits allocated to research projects and accelerate their translation to clinical space.
More than the capital, is so much time that it takes to find good suppliers that are willing to make the products as I think its best.

Regarding liposomal formulations, they can be good solution to increase bio-availability of supplements – and I would add some of those as discussed above as long as they are taken from a good supplier – but I would clearly not replace intravenous treatment with those.

Kind regards,
Daniel

嗨，Mihaela，

谢谢你。我找到了愿意帮助的人，即使目标是将大部分利润用于研究项目，并加速它们向临床领域的转化。
除了资本之外，还需要花费大量时间来寻找愿意按照我认为最好的方式制造产品的良好供应商。

关于脂质体配方，它们可以是增加补充剂生物利用度的好方法 - 我会像上面讨论的那样添加一些来自好供应商的产品 - 但我肯定不会用它们来替代静脉治疗。

亲切的问候，
Daniel

Hi Daniel,

Well I hope your supplement company is off to a good start. I can’t imagine the amount of capital it would take to start something like that, hopefully you have some good investors.

That’s a shame, I really wished liposomal drugs had better bioavailability than what they claim. Marketing <– for your supplement company. We will hold off on Vit C for now.

Thank you responding and all that you do!

Mihaela

嗨，Daniel，

希望你的补充剂公司有个良好的开端。我无法想象启动这样一个项目需要多少资金，希望你有一些好的投资者。

真是可惜，我真的希望脂质体药物的生物利用度比它们声称的更好。这也是你的补充剂公司的市场宣传内容。我们暂时不会使用维生素C。

感谢您的回复和您所做的一切！

Mihaela

Synergic Effects of Artemisinin and Resveratrol in Cancer Cells:
https://pubmed.ncbi.nlm.nih.gov/25048878/?dopt=Abstract

“The combination of the two drugs also markedly reduced the ability of cell migration. Apoptosis analysis showed that combination of ART and Res significantly increased the apoptosis and necrosis rather than use singly.

Additionally, ROS levels were elevated by combining ART with Res.”

合作作用的青蒿素和白藜芦醇在癌细胞中的效果：

https://pubmed.ncbi.nlm.nih.gov/25048878/?dopt=Abstract

“这两种药物的结合也显着降低了细胞迁移的能力。凋亡分析表明，与单独使用相比，青蒿素和白藜芦醇的组合显着增加了凋亡和坏死。

此外，将青蒿素与白藜芦醇结合会增加ROS水平。”

HI Mihaela,

Thank you so much. The high traffic indeed generates costs and not profits. Donations like yours help keep this running. I am now trying to convert this in a sustainable way for this kind of knowledge and be able to do much for for people without expecting donations. That is by (very soon) starting up a supplement company, which is my last and probably successful try to continue this in a sustainable way.

Thanks a lot for sharing your knowledge on Art. That is very helpful.

Regarding Vitamin C liposomal, I do not see that as an alternative to intravenous. But here is an idea how you could try to achieve that with oral Vitamin C, by combining DHA +Vitamin C + Liposomal Vit C https://www.cancertreatmentsresearch.com/community/postid/2747/

During the past years I looked a lot into Liposomal options. Also now when starting up the supplement company I had to look into that to select partners to work with. I even had a deep discussion about this a few hours ago with a friend who is an expert in this. The conclusion is that Liposomal oral formulations are these days coming from so many different type of suppliers and most of them are not having the right quality and profile to induce 1. a high blood level of the drug 2. maintain a level that reaches tumors. First depends on the quality and second specifically on the formulation of liposomes since most of them will be eaten up by macrophages before getting to the tumor. PEG formulations are less affected by macrophages.
So when it comes to oral formualstions my rule is like this ~75% of target dose use non-liposomal products and if budget allows ~25% use liposomal or other formulations that promise much higher absorption (the second will be a lottery ticket in case the product will rely deliver what is promised).
So, I like liposomal but what is commercially available is questionable. And since here we deal with a matter of life, we do not want to fully depend on the promise of various food supplement companies. Intravenous formulations on the other hand can be often very relevant.

Yes, Mihaela, Jane’s book is a good start into the world of repurposed drugs and metabolic cocktails. And it’s great to hear that the results are showing improving effectiveness. After all these years, to me is clear that 1. metabolism is one of the most important perspective to address cancer in a more controllable way 2. cocktails of drugs and supplements (oral and IV) help extend and improve life.

Thank you!

Kind regards,
Daniel

嗨，米哈埃拉，

非常感谢。高流量确实产生成本，而不是利润。像您这样的捐赠帮助我们保持运营。我现在正在尝试以一种可持续的方式将此转化为这种知识，并能够为人们做更多事情而不期望捐赠。那就是（很快）开始一家补充剂公司，这是我最后一次也可能是成功的尝试，以可持续的方式继续下去。

非常感谢您分享有关青蒿素的知识。这非常有帮助。

关于脂质体维生素C，我不认为它是静脉注射的替代品。但是，这里有一个想法，您可以尝试用口服维生素C实现这一目标，方法是结合DHA + 维生素C + 脂质体维生素C https://www.cancertreatmentsresearch.com/community/postid/2747/

在过去的几年里，我对脂质体选项进行了大量研究。现在，当我开始启动补充剂公司时，我不得不研究这个问题，以选择合作伙伴。几个小时前，我甚至和一位是这方面专家的朋友进行了深入的讨论。结论是，口服脂质体制剂如今来自许多不同类型的供应商，其中大多数没有正确的质量和特性，无法产生1.药物的高血浆水平2.保持能够到达肿瘤的水平。首先取决于质量，其次特别是脂质体的配方，因为大多数脂质体会在到达肿瘤之前被巨噬细胞吞噬。聚乙二醇（PEG）制剂受巨噬细胞影响较小。因此，当涉及口服制剂时，我的原则是，目标剂量的约75%使用非脂质体产品，如果预算允许，约25%使用脂质体或承诺提高吸收的其他制剂（第二种将是一张彩票，如果产品确实提供了所承诺的内容）。
因此，我喜欢脂质体，但商业上可用的产品令人怀疑。由于在这里我们处理的是生命问题，我们不希望完全依赖于各种食品补充剂公司的承诺。另一方面，静脉制剂通常是非常相关的。

是的，米哈埃拉，简的书是进入重复使用药物和代谢鸡尾酒世界的良好起点。很高兴听到结果显示出改善的有效性。经过这么多年，对我来说清楚的是，1. 代谢是以更可控的方式解决癌症问题的最重要途径之一。 2.药物和补充剂（口服和静脉注射）的混合物有助于延长和改善生命。

谢谢！

亲切的问候，
丹尼尔

Hi Daniel,

No problem, you deserve it, your work is impressive to say the least. Since you have high traffic you should definitely be making money from it not the other way around. But you probably don’t want any bad advertising and for people to buy products that are not vetted.

I interpreted how the ART got into the cytosol from the interstitial space, I don’t know why. I thought that the free iron bound to ART which in turn bound to the transferrin-receptor and together they entered the cell by endocytosis. Which makes no sense because there should not be any free iron, it’s transferrin. Then I thought well how is ART going to get out and cause ROS since the HCQ increase the pH in the vacuole not releasing ferritin, therefore not releasing ART in the cytosol either. The second mechanism of action for HCQ that you mentioned is what I was worried about. This was all in my head which is all wrong.

I found this awesome paper that explains everything.
http://microbialcell.com/researcharticles/the-molecular-and-cellular-action-properties-of-artemisinins-what-has-yeast-told-us/

First of all ART is permeable to the cell membrane, so it enters through lipid layer no problem. ART doesn’t like being in the cytosol and goes to any lipid membrane since it’s hydrophobic. I now understand why ART induces lysosomal degradation of ferritin, therefore releasing free iron in the cytosol which is toxic for the cell.

ART is reduced or activated by Fe2+, heme and Copper. Activated ART causes ROS, the apoptosis. If HQC reduces ferritin then there’s less heme to activate it, supply chain issue. But we just said that there would be more free iron in the cell from the degradation of ferritin, therefore it ART could be activated that way too for a period of time like you said.

The paper suggests there’s another mechanism of action but only for yeast and malarial mitochondria. Again attacking the lipid membrane of the mitochondria. No energy factory, cell goes bye bye. Maybe just maybe if there’s less heme and at some point less free iron to activate ART, then it can focus on the anti-mitochondrial pathway even thought the paper said mammalian cells mitochondria didn’t respond to it.

I do worry about iron deficiency anemia from the HCQ, do you suggest iron supplementation? Maybe we’ll just monitor her RBC count. Increasing iron will negate the anti-mitochondrial pathway.

I definitely don’t want to stop HCQ. Definitely synergy in the beginning both causing ROS, then later maybe ART attacks the lipid membranes including the mitochondria since there’s less activation from heme or Fe2+, where else would it go, to the lipid layer of course. This is my hope.

I thought about vitamin C for better iron absorption, but I don’t want Vitamin C rescuing the cell from ROS. Then I thought about high dose IV vitamin C but I don’t want her going out all that much with the coronavirus and it’s quite expensive treatment. I haven’t looked into liposomal vitamin C to see if bioavailability is equivalent to IV. There’s a lot of YouTube videos to make your own liposomal vitamin C as well. I wonder if we can get it high enough to generate hydrogen peroxide, that way we’d have even more ROS AND it will help the absorption of iron. How do you feel about liposomal drugs? There’s liposomal Artemisin and Curcumin, is it worth it?

I’ll look into Clarithromycin as well.

We started her on a lot of medications and supplements and ketogenic diet based off of Jane Mcclelland book – how to starve cancer. Same idea, cancer is a metabolic disease not genetic. Her last scan her biggest tumor went from 9mm to 7mm and all the other ones stayed the same so it’s working. Before that grew 1mm every 3 months. I worry about resistance and the cancer getting smart so that’s why I want to be as aggressive as possible.

Thanks for all you knowledge!

Mihaela

嗨，丹尼尔，

没问题，你值得拥有这些支持，你的工作令人印象深刻，这一点毫不夸张。由于您的网站访问量很高，您应该从中获利，而不是相反。但您可能不希望出现不良广告，以及人们购买未经验证的产品。

我误解了青蒿素是如何从间质空间进入细胞质的，但我不知道为什么。我以为游离铁结合到青蒿素上，然后与转铁蛋白受体一起通过内吞作用进入细胞。这是毫无意义的，因为不应该有游离铁，应该是转铁蛋白。然后我想，青蒿素如何才能释放出来产生ROS，因为羟氯喹增加了液泡的pH值，不释放铁蛋白，因此也不释放青蒿素。你提到的羟氯喹的第二种作用机制是我担心的。这一切都在我的头脑中，完全错误。

我找到了这篇很棒的论文，解释了一切。

首先，青蒿素可以穿透细胞膜，因此它可以轻松通过脂质层进入细胞。青蒿素不喜欢在细胞质中，而是会转移到任何脂质膜上，因为它是疏水性的。我现在明白了为什么青蒿素会诱导铁蛋白的溶酶体降解，从而在细胞质中释放游离铁，这对细胞是有毒的。

青蒿素被Fe2+、血红素和铜还原或激活。激活的青蒿素产生ROS，诱导凋亡。如果HCQ减少了铁蛋白，那么蛋白激酶的量就减少了，供应链问题。但我们刚刚说过，细胞内从铁蛋白降解产生更多的游离铁，因此青蒿素在一段时间内也可以通过这种方式被激活，就像你说的那样。

这篇论文指出了另一种机制，但仅适用于酵母和疟疾线粒体。再次攻击线粒体的脂质膜。没有能源工厂，细胞就会消失。也许只有当血红素和游离铁减少到一定程度时，青蒿素才会专注于抗线粒体途径，尽管该论文提到哺乳动物细胞的线粒体对其没有反应。

我担心HCQ会导致缺铁性贫血，您建议补充铁吗？也许我们只需监测她的红细胞计数。增加铁会抵消抗线粒体途径。

我绝对不想停止HCQ。在开始阶段肯定存在协同作用，都产生ROS，然后之后可能青蒿素攻击脂质膜，包括线粒体，因为血红素或Fe2+的激活减少了，那么它还会去哪里呢，当然是去脂质层。这是我的希望。

我考虑过维生素C以促进铁的吸收，但我不想让维生素C拯救细胞免受ROS的影

响。然后我考虑了高剂量的静脉注射维生素C，但我不希望她因新冠疫情而外出，而且这种治疗费用相当昂贵。我还没有研究过脂质体维生素C是否具有等效的生物利用度。有很多YouTube视频可以制作自己的脂质体维生素C。我想知道我们是否能使其浓度足够高以产生过氧化氢，这样我们就会有更多的ROS，并且它将帮助铁的吸收。您对脂质体药物有何看法？有脂质体青蒿素和姜黄素，值得吗？

我也会研究克拉霉素。

我们开始给她使用了大量的药物和补充剂，并根据简·麦克莱兰的书《如何让癌症挨饿》进行了基于生酮饮食。同样的理念，癌症是一种代谢性疾病，而不是遗传性疾病。她最后一次扫描显示她最大的肿瘤从9毫米变为7毫米，其他所有肿瘤都保持不变，所以这个方法是有效的。在此之前，每三个月就会增长1毫米。我担心会出现耐药性，癌细胞变得聪明，所以我想尽可能地采取积极措施。

感谢您分享所有的知识！

米哈埃拉

Dear Mihaela,

First, with this I would like to thank you for the donation that helps a lot with maintaining this website which due to high traffic requires a good amount of resources to keep it up and running.

Your question is of-course a very good one. Here is how I see the mechanisms that can explain both a synergy potentials and an antagonistic activity:

HCQ inhibits autophagy leading to the interruption of degradation of damage mitochondria which in turn leads to increase ROS generation by the damage mitochondria. HCQ on the other hand also inhibits ferritin degradation in lysosomes, lowering the labile iron pool in the cytosol. https://dm5migu4zj3pb.cloudfront.net/manuscripts/115000/115301/cache/115301.1-20140626150537-covered-253bed37ca4c1ab43d105aefdf7b5536.pdf But Arte can induce lysosomal degradation of ferritin https://www.nature.com/articles/s41418-019-0352-3 that leads to increase ROS
This can lead to two different mechanisms of ROS generation that can work in synergy https://pubmed.ncbi.nlm.nih.gov/25308836/

It’s true that this can work only for a certain amount of time as long as Arte has on what to act to generate ROS. At some point there will be no ferritin left to degrade since HCQ inhibits further accumulation of iron (that can be stored in ferritin form) since iron from iron-saturated transferrin needs a low pH to be released, within the endosome, while HCQ interferes and alkalizes with endosomal.

Latter, as there is no ferritin left for Arte to do it’s job, from an anti-cancer potential we are only left with HCQ to act as an anti cancer agent.

Therefore, it’s a time dependent activity of this combo, first working in synergy towards increasing the ROS level, latter working in antagonism. Actually maybe I should not say antagonism since they do not cancel each other out, but just one of them will continue working only, i.e. HCQ.

We could also argue that this is the theoretical picture, and that in real life HCQ does not totally inhibit the iron deployment into cells and the ferritin degradation, but only slows them down.

If we stop HCQ to start Arte we may need to wait a few weeks for that to be totally out of the body, since the half-time of HCQ is some weeks.

Another strong autophagy inhibitor could be this one but it’s an antibiotic https://www.cancertreatmentsresearch.com/community/autophagy-inhibitors/clarithromycin-is-an-autophagy-inhibitor/#post-2885

A strategy along the line of ROS generation that may be relevant is discussed here https://www.cancertreatmentsresearch.com/modulating-the-yin-and-yang-energy-of-cells-to-fight-cancer-pro-oxidant-strategy/

I hope this answers your question.

Kind regards,
Daniel

亲爱的米哈埃拉，

首先，我想感谢您的捐款，这对于维护这个网站非常有帮助，由于访问量较大，需要大量资源来保持网站的正常运行。

您的问题当然是一个非常好的问题。以下是我对可能解释协同作用潜力和拮抗活性的机制的看法：

羟氯喹抑制自噬，导致损伤的线粒体的降解中断，从而增加损伤的线粒体产生的ROS。另一方面，羟氯喹还抑制溶酶体中铁蛋白的降解，降低细胞质中的可变铁池。但青蒿素可以诱导铁蛋白的溶酶体降解，导致ROS增加。这可以导致两种不同的ROS生成机制，可以协同作用。

这只能在一定时间内有效，因为只要青蒿素有可以作用的物质生成ROS，就可以产生协同作用。在某个时候，由于羟氯喹抑制了铁的进一步积累，将没有铁蛋白可供降解。因此，从抗癌的潜力来看，我们只剩下羟氯喹作为抗癌剂。

因此，这是这种组合的时间依赖性活动，首先在增加ROS水平方面协同作用，然后在拮抗作用中继续起作用。事实上，也许我不应该说是拮抗作用，因为它们不会互相抵消，而是只有其中一个会继续起作用，即羟氯喹。

我们还可以说这是理论上的情况，在现实生活中，羟氯喹并不完全抑制铁进入细胞和铁蛋白的降解，而只是减缓了这些过程。

如果我们停止羟氯喹以开始青蒿素，我们可能需要等待几周才能完全清除体内的羟氯喹，因为羟氯喹的半衰期约为几周。

另一个强力的自噬抑制剂可能是这种抗生素。

沿着ROS生成的线路可能相关的策略在这里讨论。

希望这回答了您的问题。

祝好，
丹尼尔

Hey Daniel,

My mom has stage IV cervical cancer, she has 6 small baby tumors in her lungs. They are so small they have not been able to take biopsies, but my sister and I are hypothesizing it’s her cervical cancer that metastasized to her lungs after her hysterectomy.

I’m very confused about Chloroquine and Artemisin, you said that they are synergistic? We have her on Hydroxychloroquine for autopaghy inhibition. We want to start her on Artemisin but you said in the chloroquine/hydroxychloroquine post that they may interfere with iron. They way Artemisin works is by coming into the cell via iron and wrecks havoc. How is it synergistic if the iron cannot come into the cell anymore due to Hydrocychloroquine? Wouldn’t the two drugs cancel each other out? We really want something for autophagy for when the cells are dying and the debris won’t be recycled by new cancer cells. What do you suggest?

Sorry I spent all day trying to figure this out and got nowhere,

Mihaela

嗨，丹尼尔，

我妈妈患有第四期宫颈癌，在肺部有6个小肿瘤。它们太小了，无法进行活检，但我和我姐姐认为这是她宫颈癌在子宫切除术后转移到肺部的结果。

我对氯喹和青蒿素感到非常困惑，您说它们具有协同作用？我们给她服用羟氯喹以抑制自噬。我们想给她开始青蒿素，但您在关于氯喹/羟氯喹的帖子中说它们可能会干扰铁元素。青蒿素的作用方式是通过铁进入细胞并造成破坏。如果由于羟氯喹而使铁无法进入细胞，这两种药物怎么可能具有协同作用呢？这两种药物不是会互相抵消吗？我们真的想要一些自噬的东西，以便当细胞死亡时，碎片不会被新的癌细胞循环利用。您有什么建议？

抱歉，我整天都在试图弄清楚这个问题，但一无所获。

米哈埃拉

Hi @Daniel,

I have received the liposomal quercetin today, has any sense to take high dosage of quercetin the four days that I do dried leaves plus 500 x 2 artemisinin extract?

For the next rounds I have ordered Artemix Artemisinin Complex 140mg, how many caps daily would be a good therapeutic dosage?

Thank you!

Kind regards Inaki

Inabari您好，

关于您的问题：

1. 在进行四天的干叶和 500 毫克 × 2 的青蒿素提取物治疗期间，服用高剂量的槲皮素是否有意义？
2. 下一轮治疗，我已经订购了 Artemix 青蒿素复合物 140 毫克，每天服用多少颗会是一个良好的治疗剂量？

我的建议如下：

1. 在进行干叶和青蒿素提取物治疗期间，槲皮素具有抗氧化和抗炎特性，可能会有一定的辅助作用。您可以在咨询医生后考虑服用适量的槲皮素，但请注意不要超过推荐剂量，以避免可能的副作用。
2. 对于 Artemix 青蒿素复合物 140 毫克，建议您在咨询医生后确定剂量。通常情况下，根据个体情况和疾病严重程度，每日剂量可能会有所不同。医生可以根据您的具体情况为您制定最合适的用药方案。

希望对您有所帮助！

祝好，
Inabari

Hi Daniel

Thank you.

Yes, i went through this topic in the past. We have a chance to apply garlic intratumoral injections as tumor is easily accessible , but i am not capable of nether decision nor application. May be such option could be postponed.

Kind Regards

Asaf

Asaf您好，

谢谢您的回复。

是的，我之前看过这个话题。由于肿瘤容易接触到，我们有机会进行肿瘤内注射大蒜素，但我既不能做出决定，也无法进行应用。也许这样的选择可以延迟考虑。

祝好，

Daniel

HI Asafsh,

Artesunate is already available for injection but Garlic not. However you may like to read this discussion on Garlic injections https://www.cancertreatmentsresearch.com/community/natural-substances/garlic-possible-treatment/#post-2057

Kind regards,
Daniel

Daniel您好，

阿呋喃已经可以用于注射，但大蒜素不行。不过，您可能会喜欢阅读有关大蒜注射的讨论，链接在这里：https://www.cancertreatmentsresearch.com/community/natural-substances/garlic-possible-treatment/#post-2057

祝好，
Daniel

Hi Daniel

I don’t know whether paper below is relevant to this article. But just in case below it is:
The Synergistic Anticancer Effect of Artesunate Combined with Allicin in Osteosarcoma Cell Line in Vitro and in Vivo
https://www.ncbi.nlm.nih.gov/pubmed/24083713

unfortunately both artemisia and garlic were administered IP, so this greatly impacts applicability for humans due to Garlic absorption.

嗨 Daniel

我不知道下面的论文是否与这篇文章相关。但以防万一，下面是链接：
阿呋喃与大蒜素在体外和体内结合对骨肉瘤细胞株的协同抗癌作用
https://www.ncbi.nlm.nih.gov/pubmed/24083713

不幸的是，大蒜素和青蒿素都是通过腹腔注射给予的，这对于人类的适用性有很大影响，因为大蒜的吸收方式不同。

Dear Daniel,

Thank you very much for the tremendous support and encouragement along the way!
This “project” is too hard and too complicated to deal with alone and I don’t have enough words to thank you especially and everyone who support us and advise in this amazing blog!

All the best!
Nissim

亲爱的Daniel，

非常感谢您一直以来的巨大支持和鼓励！这个“项目”太艰难、太复杂了，独自应对是不够的，我没有足够的话语来特别感谢您和所有在这个了不起的博客中支持我们和给予建议的人！

一切顺利！
Nissim

Hi Nissim,

Very nice to hear about the “no news”!

I would use Artemisia regardless of chemo or not. Cycles are a great idea indeed, specifically because it may be a little heavy on the liver when taken continuously for long time. I would use Artemisia also during the days with Curcumin, etc. but just try to take them the tea at least half an hour apart from the others if possible and on empty stomach.

All the best to you and your brother dear Nissim!

Kind regards,
Daniel

嗨，Nissim，

很高兴听到“没有消息”！

我建议无论是否进行化疗都使用艾草。周期是个好主意，特别是因为长时间连续使用可能会对肝脏造成一定负担。我建议在服用姜黄素等药物的日子也服用艾草，但尽量让它们相隔至少半小时，而且最好在空腹时服用。

祝你和你的兄弟一切顺利！

亲切的问候，
Daniel

Hi Daniel and all!
How are you?

We were quite intense this week, as my brother had another (4th) brain MRI on Sunday and analysis with his oncologist yesterday.
The MRI demonstrated stability after additional 2 chemo cycles (3 long months).
This month it will 1 year with the GBM cancer and for now NO NEWS IS GOOD NEWS!

This week we also got 200 gr of dried Artemisia annua whole plant and I have some questions about adding Artemisia into our chemo and alternative protocol:

Q1 – we plan to use it along the day (3-4 times x 250ml) as “tea” extracted with warm (not hot) water and I was thinking of using it 1 week on & 1 off. any other suggestions?

Also, as I understand Artemisia is a (strong?) prooxidant and as such I am not sure how to optimise its use in combination with chemo and our protocol:
Q2 – is it ok or even recommended to use Artemisia on the week of chemo? (1 week of TMZ + CCNU every 6 weeks)
Q3 – what about using Artemisia while taking other daily antioxidants (which we stop during the chemo)? We prefer not to stop using these while taking Artemisia, so how best to combine?
Q4 – Just to be sure, I understand that all the following, which we use regularly or alternately are antioxidants? (in some level) –
Boswellia, Curcumin, Berberine, Melatonin, Vit E, Vit D3, Omega 3, Astragalus, Milk thistle, EGCG, Bacopa monnieri, Honokiol and Ashwagandha.

Many thanks!
Wishing everyone stability and improvement of health!!!

Best regards,
Nissim

嗨，Daniel和大家！
大家好吗？

这周我们很紧张，因为我兄弟在周日进行了第四次脑部MRI检查，昨天与肿瘤学家进行了分析。
MRI显示在额外进行了2个化疗周期（3个漫长的月份）后，病情保持稳定。
这个月是他患上胶质母细胞瘤癌症的第一年，到目前为止，没有消息就是好消息！

这周我们还收到了200克干燥的艾草全草，我有一些关于将艾草添加到我们的化疗和替代方案中的问题：

问题1 - 我们计划一天使用3-4次250毫升的"茶"，用温水（不是热水）提取。还有其他建议吗？

此外，我了解到艾草是（强大的？）前氧化剂，因此我不确定如何在化疗和我们的方案中优化其使用：
问题2 - 在化疗周期中使用艾草是否可以或甚至推荐？（每6周一次的一周TMZ + CCNU）
问题3 - 在服用其他日常抗氧化剂时使用艾草如何？（我们在化疗期间停止使用这些）我们希望在服用艾草时不停止使用这些，那么最好如何结合？
问题4 - 为了确保，我了解到以下所有物质，我们定期或交替使用的物质都是抗氧化剂？（在某种程度上）-
乳香、姜黄素、小檗碱、褪黑激素、维生素E、维生素D3、欧米伽3、黄芪、水飞蓟、EGCG、植物牛膝、本草莓和灵芝。

非常感谢！
祝愿大家的健康保持稳定和改善！！！

最好的祝愿，
Nissim

Dear Nissim,

Nice to hear from you! I hope you had a good holiday too!
Thank you for sharing your concerns regarding Artemisia Annua and it’s possible impact on the effectiveness of seizure medication.
I am not aware of any such effects and I haven’t heard anyone reporting anything on this line.

Kind regards,
Daniel

尊敬的Nissim，

很高兴收到你的来信！希望你也度过了愉快的假期！
谢谢你分享对青蒿素及其可能影响癫痫药物效果的担忧。
我不知道任何这样的影响，也没有听说过任何人报告过这方面的情况。

祝一切顺利，
Daniel

Dear Daniel and all,
I hope you all enjoy the new year holiday!

I was looking further at Artemisia annua, which seems very promising and was also recommended in the 2nd opinion report we got, as another option for chloroquine (together with using Metformin).

I happen to see some warning here, about possible cause of seizures due to use of Artemisia annua:
https://www.mskcc.org/cancer-care/integrative-medicine/herbs/artemisia-annua
“Do not take if: You are taking antiseizure medications: Artemisia can induce seizures making such medications less effective.”
and…
https://www.webmd.com/vitamins/ai/ingredientmono-729/wormwood
“Seizure disorders, including epilepsy: Wormwood contains thujone, which can cause seizures. There is concern that wormwood might make seizures more likely in people who are prone to them”

looking at this “thujone”, perhaps it is more relevant to another type of Artemisia?
https://en.wikipedia.org/wiki/Thujone
“Thujone is found in a number of plants… and wormwood, most notably grand wormwood (Artemisia absinthium)”.

https://en.wikipedia.org/wiki/Artemisia_absinthium
Remark – this becomes more confusing to me, since in this page it is mentioned that “Medicinal extracts of wormwood have not been shown to cause seizure or other adverse effects at usual doses”.

So I’m not sure, is this warning less related/ not related to Artemisia annua?
Have you heard of any case of seizures due to use of Artemisia annua?

Many thanks!
Best wishes,
Nissim

尊敬的Daniel和大家，

希望大家度过了愉快的新年假期！

我进一步研究了青蒿素，这似乎非常有前途，也被建议作为氯喹的另一种选择（与使用二甲双胍一起）。

我无意中看到了一些警告，可能是由于使用青蒿素引起癫痫发作：
https://www.mskcc.org/cancer-care/integrative-medicine/herbs/artemisia-annua
“不要服用如果：你正在服用抗癫痫药物：青蒿素可能会引发癫痫发作，使此类药物的效果减弱。”
以及…
https://www.webmd.com/vitamins/ai/ingredientmono-729/wormwood
“癫痫等癫痫症状：苦艾含有白藜芦醇，可以引起癫痫。有人担心苦艾可能会使容易发作的人更容易发生癫痫”

看到这个“白藜芦醇”，也许更相关于另一种类型的青蒿？
https://en.wikipedia.org/wiki/Thujone
“白藜芦醇存在于许多植物中……和苦艾，尤其是大苦艾（Artemisia absinthium）”。

https://en.wikipedia.org/wiki/Artemisia_absinthium
注意-对我来说这变得更加混乱，因为在这个页面中提到“药用苦艾提取物在通常剂量下未显示出引起癫痫或其他不良反应”。

所以我不确定，这个警告是否与青蒿素更少关联/无关？
您有听说过由于使用青蒿素而引起癫痫发作的案例吗？

非常感谢！
祝一切顺利！
Nissim

Hi All-
Here are 3 great resources on Artesunate IV administration from Paul Anderson, who has a significant amount of clinical experience:

Artesunate Monograph- Covers initial administration building up to full dose, drip vs push, monitoring, complication and synergism with vitamin C. Also discusses iron (Anderson is of the opinion that it doesn’t need to be given unless clinically indicated due to anemia)
https://www.academia.edu/20316500/Artesunate_Monograph

Answers some additional questions on Artesunate
https://www.consultdranderson.com/iv-artesunate-considerations-cont/
https://www.consultdranderson.com/b17-artesunate-iv-vitamin-c-cont/

大家好，

以下是保罗·安德森（Paul Anderson）关于青蒿素静脉注射（IV）使用的三个优质资源，他在临床方面拥有相当多的经验：

1. 《Artesunate专论》- 包括初始使用建议，逐渐增加剂量，滴注与推注，监测，并与维生素C的协同作用。还讨论了铁剂（安德森认为除非因贫血而临床指示，否则不需要使用铁剂）
   链接：[Artesunate Monograph](https://www.academia.edu/20316500/Artesunate_Monograph)

2. 回答了一些有关Artesunate的额外问题
   链接：[Answers some additional questions on Artesunate](https://www.consultdranderson.com/iv-artesunate-considerations-cont/)
   链接：[Answers some additional questions on Artesunate](https://www.consultdranderson.com/b17-artesunate-iv-vitamin-c-cont/)

希望这些资源能对您有所帮助。

Hi Johan,
It seems there are cases of ART compound efficacy both used with and without iron, but there is no doubt that maximizing the iron in the cancer cell increases efficacy. This is countered with the concern that too much iron in the serum and RBCs could reduce efficacy and cause RBC lysis. I have been giving my husband a 25mg iron injection once a month to help keep his ferritin and serum iron in the normal range, it also gives his hemoglobin a boost. I’m sure the cancer cells are also grabbing on to that same iron and storing it up. I read through the paper you linked to and info from D and, after some deliberation, I think we are going to try the artesunate initially without an iron supplement since the iron injection may be loading the cells, and see if we get a response. We will change our strategy if needed. My husband has done oral artemisinin combined with whole plant in the past, but more than 2 days in a row cause stomach upset. We probably won’t start this for a few more weeks as we wait for the medication to arrive. As always, thank you for taking the time to help and provide useful information, tips and important aspects I hadn’t considered.
Warmly,
Shanti

Hi Johan,

看起来有使用铁剂和不使用铁剂的ART化合物有效性的案例，但毫无疑问，最大限度地增加癌细胞中的铁剂会增加其功效。但这也引发了另一个担忧，过多的血清和红细胞中的铁剂可能会降低效果并导致红细胞溶解。我每个月给我丈夫注射25毫克的铁剂，以帮助保持他的铁蛋白和血清铁处于正常范围，这也提高了他的血红蛋白含量。我相信癌细胞也在吸收同样的铁剂并将其储存起来。我阅读了你链接的论文以及D提供的信息，在一番思考后，我认为我们将首先尝试使用artesunate（青蒿琥脂），不使用铁剂补充剂，因为铁剂注射可能会使细胞负荷加重，然后观察是否有反应。如果需要，我们会改变策略。我丈夫过去曾经口服过与整株植物结合的青蒿素，但连续超过两天会导致胃不适。我们可能会等几个星期再开始，等待药物到货。一如既往，感谢您抽出时间提供帮助和有用的信息，提示以及我没有考虑到的重要方面。

热情地，
Shanti

Shanti, regarding iron admin prior to ART treatment:
“I have been unable to give up thinking about the man with prostate cancer in Belgium who tried a unique approach to treatment with ART compounds. One day a week he took a supplemental iron pill with one meal and then took 700 mg of ART compounds that evening. He reported a substantial reduction in his PSA with this unique treatment approach. Unfortunately, there is no contact information to follow up on his long term results with this innovative approach. ”
http://www.medicinacomplementar.com.br/biblioteca/pdfs/Cancer/ca-1735.pdf

Shanti，关于在ART治疗前给予铁剂：

“我一直无法忘记比利时患前列腺癌的男士尝试了一种独特的ART化合物治疗方法的情况。每周的一天，他在一顿饭中服用了一片铁剂，然后在晚上服用了700毫克的ART化合物。他报告说，通过这种独特的治疗方法，他的PSA大幅减少了。不幸的是，没有联系信息可以跟进他使用这种创新方法的长期效果。”

 原文链接 http://www.medicinacomplementar.com.br/biblioteca/pdfs/Cancer/ca-1735.pdf

Hi Daniel, could you check on the spam folder, I think my response to Shanti should be in there

嗨，丹尼尔，你能检查一下垃圾邮件文件夹吗？我认为我对Shanti的回复可能在那里。